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well plates  (Celprogen Inc)


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    Celprogen Inc well plates
    Well Plates, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+oligodendrocytes/Human+Oligodendrocyte+Primary+Cell+Culture+Extra-cellular+Matrix/pm40095747-78-14-20
    Average 93 stars, based on 3 article reviews
    well plates - by Bioz Stars, 2026-10
    93/100 stars

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    Expressing:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Injection:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Marker:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Cell Culture:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Control:

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission
    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well. .. T

    Article Title: Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.
    Article Snippet: Primary human oligodendrocytes (36055- 22, Celprogen, Torrance, CA, United States) were seeded into 24- well plates (E36055- 22- 24 Well, Celprogen) at a density of 1 × 105 cells/well.. These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.These cells were cultured in complete growth media (M3605522S, Celprogen) under 5% CO2 with a high atmospheric humidity (90%–95%) at 37°C.

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: collected is for MITOTRACKER® (red channel) and beta-catenin (green channel). .. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen). .. Cells are weaned off with fetal bovine serum containing differentiation medium (Ce

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: are treated for 24 hrs, after which images are captured of live cells using an inverted Zeiss microscope. .. The protective effects of a test compound from Cpz toxicity are determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) is incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochond

    Article Title: Pharmaceutical compositions comprising one or more pyrone compounds, and their use for treating inflammatory and neurodegenerative diseases
    Article Snippet: Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).Data collected is for MITOTRACKER® (red channel) and beta-catenin (green channel).. The protective effects of a test compound from Cpz toxicity is determined using primary human oligodendrocytes, which are grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells are weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: alone is toxic to the human oligodendrocytes, wherein combination with compound A1 and cuprizone appears to be protective at various dosings. .. The protective effects of compound A1 from cuprizone toxicity was also determined using primary human oligodendrocytes (Celprogen) and followed with MITOTRACKER®. .. MITOTRACKER® (100 nM) was incubated with the live cells at 37° C. for 30 min. MITOTRACKER® labeling of mitochondria was assessed using a Zeiss inverte

    Article Title: Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
    Article Snippet: The results are shown in FIG. 6.The results are shown in FIG. 6.. The protective effects of compound A1 from cuprizone toxicity was determined using primary human oligodendrocytes, which were grown and differentiated in 6 well dishes coated with human oligodendrocyte extracellular matrix (ECM) (Celprogen).. Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.Cells were weaned off with fetal bovine serum containing differentiation medium (Celprogen) up to 25 days in culture.



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    Celprogen Inc 24 well plates
    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in <t>oligodendrocytes</t> in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.
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    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in <t>oligodendrocytes</t> in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.
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    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in <t>oligodendrocytes</t> in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.
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    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in <t>oligodendrocytes</t> in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.
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    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in <t>oligodendrocytes</t> in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.
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    CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in oligodendrocytes in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.

    Journal: Addiction Biology

    Article Title: Crystallin Alpha B Inhibits Cocaine‐Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission

    doi: 10.1111/adb.70028

    Figure Lengend Snippet: CRYAB‐expressing cell types in the cocaine‐administered mice NAcc and reduced Extracellular CRYAB levels in oligodendrocytes in stress‐induced relapse. (A) The mice are injected with cocaine (5 mg/kg [i.p.]). The mice brain sections (10‐μm‐thick) are reacted with anti‐CRYAB (green) and antieach brain cell marker (red). MBP, NeuN, GFAP and IBA1 are markers of oligodendrocytes, neurons, astrocytes and microglia, respectively. Closed arrows indicate the colocalization of each marker and CRYAB. Open arrows indicate the non‐colocalization of each marker and CRYAB. Original magnification is 200X. The scale bar is 25 μm. (B) The CRYAB protein levels are measured in culture media of primary human oligodendrocytes by using ELISA kit. Primary human oligodendrocytes are cultured in control or cocaine‐treated media (1 or 10 μM) for 24 h, followed by treatment with corticosterone (200 ng/mL) for 1 h. Data are expressed as the mean ± SE ( n = 4 for each cocaine‐treated group without corticosterone, corticosterone control and 1‐μM cocaine‐treated group with corticosterone, respectively; n = 6 for 10 μM cocaine‐treated group with corticosterone) and are analysed using two‐way repeated measure ANOVA followed by the Holm–Sidak post hoc t test (** p < 0.01 vs. each control, ## p < 0.01 vs. each cocaine at the same treatment dose). NAcc, nucleus accumbens.

    Article Snippet: Primary human oligodendrocytes (36055‐22, Celprogen, Torrance, CA, United States) were seeded into 24‐well plates (E36055‐22‐24 Well, Celprogen) at a density of 1 × 10 5 cells/well.

    Techniques: Expressing, Injection, Marker, Enzyme-linked Immunosorbent Assay, Cell Culture, Control